Raw data of the article "Bovine Whey Fractions Restore Epithelial Barrier Function Under TNF-α/IFN-γ Challenge in a Caco-2/HT29-MTX Co-Culture Model"

Description of the project Inflammation-induced intestinal barrier dysfunction is a key feature of several gastrointestinal disorders. Bovine whey contains bioactive components with potential barrier-protective and immunomodulatory properties, although its activity may depend on fraction composition. In this study, the effects of whole bovine whey (BWW) and skimmed bovine whey (BSW) were evaluated in a differentiated Caco-2/TC7:HT29-MTX co-culture model exposed to basolateral TNF-α + IFN-γ (50 ng/mL, 24 h). Cytokine treatment significantly reduced transepithelial electrical resistance (TEER), downregulated CLDN1 and CLDN3, increased IL-1β expression, and decreased MUC2 expression, whereas FITC-dextran permeability remained unchanged. Both whey fractions restored TEER to values comparable to control and also increased TEER under basal conditions. BWW attenuated cytokine-induced IL-1β upregulation, whereas BSW increased MUC2 expression under both basal and inflammatory conditions. These results indicate that BWW and BSW improve epithelial barrier function under inflammatory conditions through distinct functional activities. Description of the dataset This dataset contains the experimental data associated with Figures S2 and 1-4 of the mentioned article. Files included -Raw Data Figure-S2.xlsx :Raw data regarding TEER values for figure S2. -Raw Data Figure-1.xlsx : Raw data regarding TEER values for figure 1. -Raw Data Figure-2.xlsx : Raw data regarding FITC-dextran flux values for figure 2. -Raw Data Figure-3.xlsx : Raw data regarding qPCR values for figure 3. -Raw Data Figure-4.xlsx : Raw data regarding qPCR values for figure 4. -Processed Data Figure-S2.xlsx :Processed data regarding TEER values for figure S2. -Processed Data Figure-1.xlsx : Processed data regarding TEER values for figure 1. -Processed Data Figure-2.xlsx : Processed data regarding FITC-dextran flux values for figure 2. -Processed Data Figure-3.xlsx : Processed data regarding qPCR values for figure 3. -Processed Data Figure-4.xlsx : Processed data regarding qPCR values for figure 4. README.txt — this document项目描述 炎症诱导的肠屏障功能障碍是多种胃肠道疾病的核心特征。牛乳清（Bovine whey）含有具备潜在屏障保护与免疫调节活性的生物活性成分，但其活性可能依赖于其组分构成。本研究采用经分化的Caco-2/TC7:HT29-MTX共培养模型，以基底外侧给予50 ng/mL的肿瘤坏死因子-α（TNF-α）与干扰素-γ（IFN-γ）处理24小时，评估全脂牛乳清（BWW）与脱脂牛乳清（BSW）的作用效果。细胞因子处理可显著降低跨上皮电阻（transepithelial electrical resistance, TEER）、下调紧密连接蛋白1（CLDN1）与紧密连接蛋白3（CLDN3）的表达、上调白细胞介素-1β（IL-1β）的表达，并下调黏蛋白2（MUC2）的表达，而异硫氰酸荧光素-葡聚糖（FITC-dextran）的通透性则无明显变化。两种乳清组分均可将TEER恢复至与对照组相当的水平，且在基础培养条件下可提升TEER值。BWW可缓解细胞因子诱导的IL-1β表达上调，而BSW在基础与炎症培养条件下均可提升MUC2的表达。上述结果表明，BWW与BSW可通过不同的功能机制，在炎症状态下改善上皮屏障功能。 数据集描述 本数据集包含对应上述论文中补充图S2及图1至图4的实验数据。 包含文件 - 图S2原始数据.xlsx：对应图S2的跨上皮电阻（TEER）原始实验数据 - 图1原始数据.xlsx：对应图1的TEER原始实验数据 - 图2原始数据.xlsx：对应图2的异硫氰酸荧光素-葡聚糖（FITC-dextran）转运通量原始实验数据 - 图3原始数据.xlsx：对应图3的实时荧光定量PCR（qPCR）原始实验数据 - 图4原始数据.xlsx：对应图4的qPCR原始实验数据 - 图S2处理后数据.xlsx：对应图S2的TEER处理后实验数据 - 图1处理后数据.xlsx：对应图1的TEER处理后实验数据 - 图2处理后数据.xlsx：对应图2的FITC-dextran转运通量处理后实验数据 - 图3处理后数据.xlsx：对应图3的qPCR处理后实验数据 - 图4处理后数据.xlsx：对应图4的qPCR处理后实验数据 README.txt — 本文档