cRNA-seq of MCMV

Swiss murine embryonic fibroblasts (NIH-3T3) were infected with wild-type Smith strain mouse cytomegalovirus (MCMV) at a multiplicity of infection (MOI) of 10. Sequencing libraries were prepared using the cRNA-seq protocol from the indicated time points of infection as described by Whisnant A.W. et al, Nat. Commun (2020) MCMV infection was conducted on NIH-3T3 cells at an MOI of 10. Total RNA was isolated for sequencing using the Trizol protocol. Circularization of RNA was performed as described in Whisnant et al., Nature commun. 2020. This results in a strong enrichment of reads at transcription start sites. Two biological replicates were performed for each of the time points: 0, 2, 6, 12, 24 and 48 hpi Please note that MCMV_tss.tar.gz contains a list of transcription start sites which are determined based on both dSLAM-seq and cRNA-seq datasets.