Mitochondrial function in PBMCs of healthy donors and ME/CFS patients (CTRL + wIRA)

Our research hypothesis was that heat treatment by water-filtered infrared-A irradiation (wIRA) at 39 °C for 60 minutes would lead to alterations in mitochondrial function compared to an untreated control. PBMCs from healthy donors and ME/CFS patients were studied. The following files show the mitochondrial parameters of ATP production, basal respiration, maximal response, spare respiratory capacity and non-mitochondrial respiration as oxygen consumption rate (OCR) per 1000 cells. Data are presented as mean values calculated from a minimum of two and a maximum of eight replicates per condition. The data show a higher mitochondrial function of the measured parameters in ME/CFS patients compared to healthy donors. Heat treatment compensates for these higher values and leads to an approximation to the healthy level.

本研究的假设为：与未处理的对照组相比，采用39℃、时长60分钟的水滤红外A（water-filtered infrared-A，wIRA）辐照热处理，可改变线粒体功能。本研究对健康捐赠者及肌脑脊髓炎/慢性疲劳综合征（Myalgic Encephalomyelitis/Chronic Fatigue Syndrome，ME/CFS）患者的外周血单个核细胞（Peripheral Blood Mononuclear Cells，PBMCs）开展了分析。下述文件展示了以每1000个细胞的耗氧速率（oxygen consumption rate，OCR）为单位的线粒体相关参数，包括ATP生成量、基础呼吸速率、最大呼吸响应、储备呼吸能力以及非线粒体呼吸速率。本研究数据以均值形式呈现，每个实验条件下的重复次数最少为2次、最多为8次。数据分析结果表明，相较于健康捐赠者，ME/CFS患者的各项检测线粒体功能参数均处于更高水平；而热处理可抵消这些偏高的数值，使线粒体功能趋近于健康人群的基准水平。