RNA-Seq of deformed wing virus (DWV) infection on honey bee larvae

Honey bee larvae were infected with deformed wing virus (DWV) by using artificial larvae feeding system under laboratory condition. The larvae were collected at 4 day post inoculation (d.p.i) and then subjected to RNA extraction for RNA-Seq. High-throughput sequencing was performed on Illumina MiSeq platform with paired-end (PE) technology of 301 bp x 2 for further transcriptome analsysis.