Precise and Predictable CRISPR Chromosomal Rearrangements Reveal Principles of Cas9-mediated Nucleotide Insertion. Shou, li et al

Original gel images for: Precise and Predictable CRISPR Chromosomal Rearrangements Reveal Principles of Cas9-mediated Nucleotide Insertion. Figure 4B, 4C and 4D : In vitro cleavage by Cas9 with biotin labeled DNA duplex and agarose-gel bands for Sanger sequencing. Figure S4A, S4B and S4C : In vitro cleavage by Cas9 and its nickases with biotin labeled DNA duplex and agarose-gel bands for Sanger sequencing. Figure S1C and S1J : Western blots verify depletion of Mre11 and CtIP. Figure S2C : Western blots verify depletion of FANCD2.

本数据集对应《精准且可预测的CRISPR染色体重排揭示Cas9介导核苷酸插入原理》一文的原始凝胶电泳图像，详情如下：图4B、4C及4D：采用生物素标记DNA双链的Cas9体外切割实验，以及用于Sanger测序的琼脂糖凝胶条带；补充图S4A、S4B及S4C：采用生物素标记DNA双链的Cas9及其切口酶的体外切割实验，以及用于Sanger测序的琼脂糖凝胶条带；补充图S1C与S1J：通过蛋白质免疫印迹（Western Blot）验证Mre11与CtIP的表达敲低；补充图S2C：通过蛋白质免疫印迹验证FANCD2的表达敲低。