遇见数据集

DADA2 formatted taxonomy from GTDBr95

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Zenodo2020-12-24 更新2026-05-25 收录
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DADA2 requires taxonomy files in a specific format. This datasets are the files required to assign taxonomy using Genome Taxonomy Database (GTDB) database. GTDB release 95.0 is the latest version of database released on July 17th, 2020. GTDB-r95 contains 30,238 bacterial and 1,672 archaeal species clusters which span 194,600 genomes. FASTA file of 16S rRNA gene sequences identified within the representative genomes of bacteria (21965) and Archaea (1126) were downloaded from this link on 24-12-2020. The link provides resources for GTDB species representatives hence, limiting one sequence per organism. The sequence headers were modified according to DADA2 requirements using regular expression based replace in Notepad++ (I was too lazy to do the same through awk/sed). Files GTDBr95-Genus.fna and GTDBr95-Species.fna are to be used with assignTaxonomy and addSpecies commands of DADA2, respectively. Prepared files were checked and found compatible when run on DADA2 v1.14.0 (R v3.6.3).

DADA2 要求使用特定格式的分类学文件。本数据集即为利用基因组分类数据库(Genome Taxonomy Database, GTDB)进行分类学注释所需的配套文件。GTDB 95.0版是该数据库于2020年7月17日发布的最新版本。GTDB-r95 共包含30238个细菌类群与1672个古菌类群的物种聚类,涵盖194600个基因组。研究团队于2020年12月24日从指定链接下载了细菌(21965个)与古菌(1126个)代表性基因组中注释得到的16S rRNA基因序列的FASTA格式文件。该链接仅提供GTDB物种代表性序列资源,因此每个物种仅对应一条序列。研究人员通过Notepad++的正则表达式替换功能,按照DADA2的要求对序列标题进行了修改(因嫌通过awk/sed工具处理过于繁琐)。其中GTDBr95-Genus.fna与GTDBr95-Species.fna文件需分别配合DADA2的assignTaxonomy与addSpecies命令使用。经测试,本数据集文件在DADA2 v1.14.0(搭配R v3.6.3)环境下可正常兼容运行。

提供机构:
Zenodo
创建时间:
2020-12-24
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