Data from: Changes in the bacterial microbiota in gut, blood, and lungs following acute LPS instillation into mice lungs

AbstractIntroduction: Previous reports have shown that the gastrointestinal (GI) bacterial microbiota can have profound effects on the lungs, which has been described as the “gut-lung axis”. However, whether a “lung-gut” axis exists wherein acute lung inflammation perturbs the gut and blood microbiota is unknown. Methods: Adult C57/Bl6 mice were exposed to one dose of LPS or PBS instillation (n = 3 for each group) directly into lungs. Bacterial microbiota of the bronchoalveolar lavage fluid, blood, and cecum were determined using 454 pyrotag sequencing and quantitative polymerase chain reaction (qPCR) at 4 through 168 hours post-instillation. We then investigated the effects of oral neomycin and streptomycin (n = 8) on the microbiota at 4 and 24 hours post LPS instillation versus control treatment (n = 5 at baseline and 4 hours, n = 7 at 24 hours). Results: At 24 hours post LPS instillation, the total bacterial count was significantly increased in the cecum (P<0.05); whereas the total bacterial count in blood was increased at 4, 48, and 72 hours (P<0.05). Antibiotic treatment reduced the total bacteria in blood but not in the cecum. The increase in total bacteria in the blood correlated with Phyllobacteriaceae OTU 40 and was significantly reduced in the blood for both antibiotic groups (P<0.05). Conclusion: LPS instillation in lungs leads to acute changes in the bacterial microbiota in the blood and cecum, which can be modulated with antibiotics., Usage notessequence_data454 sequencing files after shhh.flows and trim,seqs commands have been implemented. No chimera removal has been done yet on these files. Their are also group files and name files associated with each respective fasta file.

摘要与引言：已有研究表明，胃肠道（GI）细菌菌群可对肺部产生显著影响，这一现象被称为“肠-肺轴”。然而，是否存在“肺-肠轴”，即急性肺部炎症会扰动肠道与血液菌群，目前仍不明确。方法：本研究以成年C57/Bl6小鼠为实验对象，经肺部直接滴注单剂量脂多糖（LPS）或磷酸盐缓冲液（PBS），每组设置3只小鼠。于滴注后4至168小时，采用454焦磷酸测序与定量聚合酶链反应（qPCR）检测支气管肺泡灌洗液、血液及盲肠的细菌菌群组成。随后，本研究对比了对照组（基线时n=5，滴注后4小时n=5，24小时n=7）与口服新霉素和链霉素组（n=8）在脂多糖滴注后4小时及24小时的菌群变化。结果：脂多糖滴注后24小时，小鼠盲肠的细菌总计数显著升高（P<0.05）；血液中的细菌总计数则在滴注后4、48及72小时均显著升高（P<0.05）。抗生素处理可降低血液中的细菌总量，但对盲肠菌群无显著影响。血液中细菌总数的升高与叶杆菌科OTU 40显著相关，且两个抗生素处理组的血液该指标均显著降低（P<0.05）。结论：肺部脂多糖滴注可引发血液与盲肠的细菌菌群发生急性变化，且该变化可通过抗生素进行干预调控。使用说明：本数据集的454测序数据文件已通过shhh.flows与trim,seqs命令完成处理，但尚未进行嵌合体去除操作。每个对应的fasta文件均配有相应的分组文件与名称文件。