Crystal structure of alpha/beta-hydrolase macrolide esterase EstX from Escherichia coli (S102A mutant) in complex with linearized tylosin

Crystal structure of alpha/beta-hydrolase macrolide esterase EstX from Escherichia coli (S102A mutant) in complex with linearized tylosin Descriptor: (3~{R},4~{S},5~{S},6~{R},8~{R},10~{E},12~{E},14~{R},15~{R})-14-[[(2~{R},3~{R},4~{R},5~{R},6~{R})-3,4-dimethoxy-6-methyl-5-oxidanyl-oxan-2-yl]oxymethyl]-5-[(2~{R},3~{R},4~{R},5~{S},6~{R})-4-(dimethylamino)-5-[(2~{S},4~{R},5~{S},6~{S})-4,6-dimethyl-4,5-bis(oxidanyl)oxan-2-yl]oxy-6-methyl-3-oxidanyl-oxan-2-yl]oxy-4,8,12-trimethyl-3,15-bis(oxidanyl)-9-oxidanylidene-6-(2-oxidanylideneethyl)heptadeca-10,12-dienoic acid, alpha/beta-hydrolase macrolide esterase EstX Authors: Kelly, E.T.R, Blanchet, J, Berghuis, A.M. Deposit date: 2026-03-12 Release date: 2026-04-08 Last modified: 2026-07-29 Method: X-RAY DIFFRACTION (1.65 Å) Cite: Hydration and hydrolysis define antibiotic resistance conferred by macrolide esterases. Proc.Natl.Acad.Sci.USA, 123, 2026