Single-cell transcriptomics reveals etiology-specific T-cell heterogeneity in hepatocellular carcinoma and implicates regulatory

We performed single-cell RNA sequencing (scRNA-seq) on CD45+ immune cells isolated from peripheral blood mononuclear cells (PBMCs), tumor tissues (T), and peritumor tissues (PT) of HBV-HCC (PBMC: n=4; T: n=3; PT: n=3) and MASH-HCC (PBMC: n=4; T: n=2; PT: n=2) patients. Integrative bioinformatics analyses were conducted to characterize T cell subsets, their functional states, and intercellular crosstalk. Validation induced multiplex immunohistochemistry and Flow cytometry on patients.