scRNA sequencing of mice with or without murine astrovirus infection undergoing cage change.

Viral persistence can be disrupted by preventing re-exposure and activating immunity upon stress recovery, indicating that external factors, such as cage changes, can be manipulated to shorten the duration of a viral infection. Laboratory mice (C57BL/6J) with and without murine astrovirus infection that did or did not undergo a cage change (2 mice per group). After 1 day, ileum cells were harvested. Cells from 2 mice per condition were pooled and then used on individual lanes for sequenceing.