Next Generation Sequencing Facilitates Quantitative Analysis of pancreatic tissue transcriptomes from KrasG12D;Pdx1-Cre and miR-301a-/-;KrasG12D;Pdx1-Cre mice treated with caerulein

Purpose: we used next generation sequencing to analyze gene expression profiles of pancreatic tissues from KrasG12D;Pdx1-Cre and miR-301a-/-;KrasG12D;Pdx1-Cre mice treated with caerulein. The goals of this study are to compare the different gene expression profiles of pancreatic tissue between KrasG12D;Pdx1-Cre and miR-301a-/-;KrasG12D;Pdx1-Cre mice treated with caerulein. KrasG12D;Pdx1-Cre and miR-301a-/-;KrasG12D;Pdx1-Cre mice were administered 8-hourly intraperitoneal injection of 50μg/kg caerulein. Mice were sacrificed at 7 days after the first caerulein injection and total RNA was extracted. mRNA profiles were generated by deep sequencing by using novaseq 6000 sequencing platform. The reads were mapped to the mouse reference genome (GRCm38) by STAR software (Version 2.5.1), annotation from GENCODE version vM21 was used. Differential gene expression was performed using DESeq2. qRT–PCR validation was performed using SYBR Green assays