A comprehensive atlas of pig RNA editome across 23 tissues reveals RNA editing affecting interaction mRNA-miRNAs
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Figure S1: PCA analysis of all tissues. Figure S2: wild(CFLAR-A) and experimental(CFLAR-G) plasmid by PCR and Sanger sequencing. Figure S3: Validation of RNA editing by PCR and Sanger sequencing. Table S1: Information of all samples. Table S2: The filtering criteria for sites of all tissues. Table S3: Primer information for RNA editing validation. Table S4: Primer information of vector construction. Table S5: Information of all RNA editing sites. Table S6: Information of all RNA-seq expression data. Table S7: Specificity of A-to-I editing sites in skeletal muscle of pigs. Table S8: Specificity of A-to-I editing sites of skeletal muscle with GO annotation. Table S9: The result of the prediction of miRNA target regions by miranda software.
补充图S1:所有组织的主成分分析(Principal Component Analysis,PCA)。 补充图S2:野生型(CFLAR-A)与实验型(CFLAR-G)质粒的聚合酶链式反应(Polymerase Chain Reaction,PCR)及桑格测序(Sanger Sequencing)结果。 补充图S3:基于聚合酶链式反应与桑格测序的RNA编辑(RNA editing)验证结果。 补充表S1:所有样本的信息。 补充表S2:所有组织的位点筛选标准。 补充表S3:RNA编辑验证所用引物信息。 补充表S4:载体构建所用引物信息。 补充表S5:所有RNA编辑位点的信息。 补充表S6:所有RNA测序(RNA-seq)表达数据的信息。 补充表S7:猪骨骼肌中A-to-I RNA编辑位点的特异性分析结果。 补充表S8:带有基因本体(Gene Ontology,GO)注释的骨骼肌A-to-I RNA编辑位点特异性分析结果。 补充表S9:通过Miranda软件预测的微小RNA(microRNA,miRNA)靶区域结果。



