Comparative Genomics of Microsporidia

The project is to sequence 13 species/isolates from the Microsporidia community. The proposed sequencing model uses 454 single reads to generate contigs and 454 paired-end reads from a jumping library to link these into larger scaffolds. 3kb jumps will be used to generate 15-fold coverage in 454 fragment reads and 30-fold physical coverage in paired 454 reads. Each genome will require 1/2 to one full 454 sequencing run. The Microsporidia are: Encephalitozoon cuniculi Anncallia algerae Edhazardia aedis Enterocytozoon bieneusi Nematocida parisii Nucleospora salmonis Pseudoloma neurophilia Paranucleospora theridion Vavraia culicis Vittaforma corneae