Data from: Rise of the machines – recommendations for ecologists when using next generation sequencing for microsatellite development.

Next generation sequencing (NGS) is revolutionizing molecular ecology by simplifying the development of molecular genetic markers, including microsatellites. Here we summarize the results of the large scale development of microsatellites for 54 non-model species using NGS and show there are clear differences amongst plants, invertebrates and vertebrates for the number and proportion of motif types recovered that are able to be utilised as markers. We highlight that the heterogeneity within each group is very large. Despite this variation, we provide an indication of what number of sequences and consequent proportion of a 454 run are required for the development of 40 designable, unique microsatellite loci for a typical molecular ecological study. Finally, to address the challenges of choosing loci from the vast array of microsatellite loci typically available from partial genome runs (average for this study, 2341 loci), we provide a microsatellite development flowchart as a procedural guide for application once the results of a partial genome run are obtained.

下一代测序（Next Generation Sequencing, NGS）正通过简化包括微卫星（microsatellites）在内的分子遗传标记（molecular genetic markers）的开发流程，革新分子生态学领域。本研究总结了利用下一代测序技术针对54种非模式物种大规模开发微卫星标记的研究成果，并发现植物、无脊椎动物与脊椎动物之间，可作为标记的基序类型的数量与占比存在显著差异。我们同时指出，每一类群内部的异质性同样极高。尽管存在上述差异，我们仍为典型分子生态学研究中开发40个可设计的独特微卫星位点所需的测序序列数量，以及由此占454测序运行（454 run）的比例，提供了参考依据。最后，为应对从部分基因组测序运行（本研究平均产出2341个位点）通常可获得的海量微卫星位点中筛选目标位点的挑战，我们提供了一套微卫星开发流程图，作为获取部分基因组测序结果后的实操指导方案。