File S1 - FRET-FLIM Investigation of PSD95-NMDA Receptor Interaction in Dendritic Spines; Control by Calpain, CaMKII and Src Family Kinase

Contains the following files: Figure S1. Representative fluorescence decay curves obtained with high or low photon count demonstrate equally good fitting characteristics; the relationship between the number of photons per pixel and the calculated lifetime is different for GluN1-GFP alone vs GluN1-GFP/PSD95-mCherry. Figure S2. GluN1-GFP/PSD95-mCherry are suitable probes to detect NMDAR/PSD95 interaction both in live and fixed HEK293 cells. Figure S3. Scatter plots of representative data for live and fixed neurons. Figure S4. Expression of PSD95-mCherry and Homer-mCherry in DIV14 live hippocampal neurons. Figure S5. Synaptic GluN2A/GluN2B ratio increases during development in cultured hippocampal neurons. Supplementary methods, immunocytochemistry, related to Figure S5. (PDF)