Transcriptome analysis of wild type and RAR deficient frog faces during development

We utilized RNASeq to investigate the changes in mRNA expression when retinoic acid signaling is inhibited by treatment with an RAR inhibitor, BMS453. Overall design: Embryos were treated with RAR inhibtior during the latter stage of facial development, 35-66 hours post fertilzation, facial region was dissection and global changes in gene expression were assessed. Further analyses to determine funcitonal networks that were affected was performed.