Bone morphogenetic protein-9 is a prognostic biomarker in outcome prediction and host_x0002_directed therapy target for sepsis

NOT PROVIDED; REQUESTED male C57BL/6 mice aged 6-8 weeks were anesthetized intraperitoneally (i.p.) with a mixture of xylazine (4.5 mg/kg) and ketamine (90 mg/kg), and the cecum was exposed, ligatured at its external third, and punctured through with a 24-gauge needle (nonsevere CLP, 50~60% survival) or a 21-gauge needle (severe CLP, 0~20% survival).Murine recombinant BMP9 (catalog 5566-BP, R&D Systems) was injected intraperitoneally at 25 μg/kg after CLP, and 4 mM HCl containing 0.1% bovine serum albumin as Vehicle was delivered in a similar fashion as control solutions. The peritoneallavagefluids were obtained at 24h after CLP,then send to Single-cell RNA sequencing.