Understanding the role of miRNA-143 (miR-143) in ischemia-induced retinopathy via RNA transcriptome profiling

To investigate molecular network mediated by miR-143, we analyzed the transcriptome profile of retinas isolated from neonatal pups subjected to either normoxia or oxygen-induced retinopathy (OIR) for 14 days (P14) receiving an intravitreal injection of either scrambled miRNAs (control) or miR-143 mimics. Total RNAs were isolated from the retinas and subjected to RNA-Seq. Analysis of RNA-Seq data identified 2064 genes with altered levels between OIR rats and shame rats receiving scrambled RNAs (adjusted p<0.05). Overall design: Four groups of samples were analysed: 1) OIR with scrambled RNA (n=3) 2) OIR with miR-143 mimic (n=3) 3) Sham with scrambled RNA (n=3) 4) Sham with miR-143 mimic (n=3)