Muller-glia targeted SMART-seq in MNU injured retina

We further investigated injury-induced gene expression changes selectively in MG cell lineages. The tdTom-labeled MG and their descendant cells were isolated via FACS from uninjured or MNU-injured Glast-CreERT;R26tdTom/+ mouse retinas, which were infected with either AAV2-Ctrl Ab or AAV2-alpha Prox1, and mRNA expression patterns of individual cells were analyzed using SMART (Switching Mechanism At the 5' end of RNA Template) sequencing methods. We could identify a lineage flow from resting MG to RPC-like cells via activated MG, along with pseudo-time progression.