Homo sapiens KBM-7 Whole genome sequencing. Homo sapiens

Gene-trap virus was produced in 293T cells by transfection of gene-trap vectors combined with pAdvantage, CMV-VSVG and Gag-pol. Approximately 100 million near haploid KBM-7 cells were infected and subsequently expanded. Cells were exposed to MG132 or Velcade and resistant clones were expanded and pooled. From the pooled clones genomic DNA was isolated and used to map insertion sites using inverse PCR. Mutations that were predicted to be disruptive in genes were counted per gene and compared to mutation frequencies in the same gene in a non-selected contra, cell population. Genes significantly enriched for mutations in the selected cell population were identified.