Tyrosine phosphorylation controlled poly(A) polymerase I activity regulates general stress response in bacteria

We did Genome wide RNA seq analysis between wild type MG1655 cells and mutant of bacterial poly(A) polymerase I (MG-pcnB null mutant). We show that pcnB-null mutation widely stabilises stress response mRNAs (>30%) and imparts cellular tolerance to multiple stresses. E.coli K-12 MG 1655 cells and MG-pcnB null mutant cells were grown up to OD600=0.5 and isolated total RNA. rRNA depleted samples were used further for cDNA library preparation and analysis