Kinetic constants for different VHH determined by Biacore binding assays.

Association rate constants (ka), dissociation rate constants (kd), equilibrium association constants (and equilibrium dissociation constants (KD = kd/ka). Independent experiments: n = 1, 2, 3. 95% CI expresses SD between independent experiments, otherwise, the SE of the fit was computed to estimate the apparent affinities for the interactions. Kinetic constants for different VHH determined by Biacore binding assays.