supplementary materials: Gentamicin sponge for anti-infection, a controversial old topic and preliminary exploration of the solution, in vitro and in vivo experiments

supplementary materials: Gentamicin sponge for anti-infection, a controversial old topic and preliminary exploration of the solution, in vitro and in vivo experiments. abstract: Although gentamicin sponge is approved for preventing or treating surgical infection in many countries, the effectiveness of it has still been controversial. Our study was to construct drug-loading and drug-release quantitative equation of gentamicin sponge, in addition, obtain the wound infection prevented and treated scheme of gentamicin sponge. Firstly, sponge was cut into 1×1×0.5cm size and immersed into 40mg/ml, 16mg/ml, 8mg/ml, 4mg/ml, 1.6mg/ml, 0.8mg/ml or 0mg/ml gentamicin solution to evaluate gentamicin-loading of sponge. Secondly, different air-dried gentamicin-saturated sponge was immersed into 10ml 0.9% physiological saline to analyze the drug-release of gentamicin sponge. Thirdly, methicillin sensitive Staphylococcus aureus (MSSA) and Pseudomonas aeruginosa (P. aeruginosa) were used to explore the infection prevented scheme of gentamicin sponge. Finally, femur fractured with wound infection rat model was used to discuss the infection treated scheme. Then, the equation of gentamicin-loading of sponge was: z=(0.03718±0.01672)x+(-4.578e-4±0.06253)y+(-2.50935e-4±1.47521e-4)x2+(0.00303±0.00149)y2+(0.00408±3.52827e-4)xy (R2 was 0.97) and drug-release equation was z=(4.37205±1.18048)x+(-7.05921±3.09628)y+(-0.04596±0.01287)x2+(0.3309±0.07912)y2+(0.31559±0.02754)xy (R2 was 0.95). 1.6 mg/ml air-dried sponge and 0.8mg/ml air-dried sponge were enough to prevent wound infection. Besides, if wound were confirmed to be sensitive bacteria infected, we recommended to use 40mg/ml air-dried sponge, 16mg/ml air-dried sponge or 8mg/ml air-dried sponge to treat.

补充材料：庆大霉素海绵（Gentamicin sponge）抗感染研究——一项颇具争议的老旧课题及其解决方案初探，包含体外与体内实验。摘要：尽管庆大霉素海绵已在多国获批用于预防或治疗手术部位感染，但其临床有效性仍存在广泛争议。本研究旨在构建庆大霉素海绵的载药与释药定量方程，并明确其伤口感染防治方案。实验步骤如下：首先，将海绵切割为1×1×0.5cm规格，分别浸没于40mg/ml、16mg/ml、8mg/ml、4mg/ml、1.6mg/ml、0.8mg/ml及0mg/ml的庆大霉素溶液中，以评估海绵的载药量；其次，将不同干燥状态的庆大霉素饱和海绵浸没于10ml 0.9%生理盐水中，分析庆大霉素海绵的药物释放特性；第三，采用甲氧西林敏感金黄色葡萄球菌（Methicillin sensitive Staphylococcus aureus，MSSA）与铜绿假单胞菌（Pseudomonas aeruginosa，P. aeruginosa）探究庆大霉素海绵的伤口感染预防方案；最后，构建股骨创伤感染骨折大鼠模型，探讨其感染治疗方案。本研究得到的海绵载药量定量方程为：z=(0.03718±0.01672)x+(-4.578e-4±0.06253)y+(-2.50935e-4±1.47521e-4)x²+(0.00303±0.00149)y²+(0.00408±3.52827e-4)xy（决定系数R²=0.97）；释药定量方程为：z=(4.37205±1.18048)x+(-7.05921±3.09628)y+(-0.04596±0.01287)x²+(0.3309±0.07912)y²+(0.31559±0.02754)xy（决定系数R²=0.95）。研究结果显示，1.6mg/ml干燥海绵与0.8mg/ml干燥海绵即可有效预防伤口感染。此外，若确认伤口为敏感菌感染，推荐使用40mg/ml、16mg/ml或8mg/ml干燥海绵进行感染治疗。