Siwiec et al 2024 - RAW full microscopic images; DG PV-SOM-GFP staining in Htr7-GFP mice

The archive contains raw microscopic images in Zeiss (.czi) format obtained during the Siwiec 2024 study (https://www.biorxiv.org/content/10.1101/2024.09.17.613425v1). Datafiles contain Z-stacks of microscopic images with metadata and four image channels (immunostaining for parvalbumin, somatostatin and GFP, with DAPI nuclear counterstain; see describing work for technical details) of transverse hippocampal sections from transgenic Htr7-GFP mice Tg(Htr7-EGFP)ST29Gsat. Example open software supporting .czi files: FIJI, Bio-Formats, ZEISS libczi C++ library (github.com/ZEISS/libczi) and its implementations (e.g. aicspylibczi in Python).本存档包含Siwiec 2024研究中获取的蔡司（Zeiss）.czi格式原始显微图像（研究链接：https://www.biorxiv.org/content/10.1101/2024.09.17.613425v1）。数据文件包含带有元数据的显微图像Z轴堆叠序列，以及来自转基因Htr7-GFP小鼠Tg(Htr7-EGFP)ST29Gsat的海马横向切片的四个成像通道：分别为针对小白蛋白、生长抑素的免疫染色通道、GFP荧光标记通道以及DAPI细胞核复染通道；技术细节详见相关研究工作。支持.czi格式文件的开源软件示例包括：FIJI、Bio-Formats、蔡司ZEISS libczi C++库（仓库地址：github.com/ZEISS/libczi）及其衍生实现（如Python环境下的aicspylibczi工具包）。