Enhancer looping protein LDB1 regulates hepatocyte gene expression by cooperating with liver transcription factors

To study LDB1 function in liver cell, gene expression changes and LDB1 binding and H3K27ac modification were detected by RNA-seq and ChIP-seq. To study how LDB1 affect gene expression in liver cells, RNA-seq were performed in control and LDB1 knockout human HepG2 cell and primary mouse liver cell. LDB1 ChIP-seq was performed in HepG2 cell to obtain genome-wide LDB1 binding. H3K27ac ChIP-seq was performed in control and LDB1 knockout HepG2 cell to study how LDB1 knockout affect enhancer mark.