COS7 pNLGFP INF-alpha

Tilt Series Date: 2012-01-20 Data Taken By: Cora Woodward Species / Specimen: Cercopithecus aethiops (monkey, African green) Strain: COS-7 ATCC CRL-1651 Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x. Acquisition Software: UCSF tomo Upload Method: rundir Processing Software Used: imod Collaborators and Roles: pNLGFP proviral construct provided by Irvin Chen UCLA. Sample prepared and imaged at Caltech by Cora Woodward Purification / Growth Conditions / Treatment: whole cells grown on grids and transfected with lipofectamine Sample Preparation: manual blotting

倾斜序列（Tilt Series）采集日期：2012年1月20日；数据采集者：科拉·伍德沃德（Cora Woodward）；物种/标本：非洲绿猴（Cercopithecus aethiops，African green monkey）；细胞株：COS-7（ATCC CRL-1651）。倾斜序列设置：采用单轴倾斜模式，倾斜范围为(-60.0°, 60.0°)，步长1.0°，角度增量恒定；电子辐射剂量为150.0 eV/Ų，欠焦值-6.0 μm，放大倍数22500×。采集软件：UCSF tomo；数据上传方式：rundir；所用图像处理软件：imod。合作者与职责分工：pNLGFP原病毒构建体（proviral construct）由加州大学洛杉矶分校的欧文·陈（Irvin Chen）提供；样品由科拉·伍德沃德于加州理工学院完成制备与成像。纯化、培养条件及处理方式：全细胞培养于电子显微镜载网（grids）之上，并通过脂质转染试剂（lipofectamine）进行转染。样品制备方法：手工印迹法（manual blotting）。