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Purification of colony-spreading stimulatory factor in calf serum.

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https://figshare.com/articles/dataset/_Purification_of_colony_spreading_stimulatory_factor_in_calf_serum_/1028408
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Calf serum (19 ml) was used as the starting medium. To measure the colony-spreading stimulatory activity, samples were serially diluted 2-fold and applied to 20 ml of soft agar and incubated for 8 h at 37°C. The diameters of giant colonies were measured from each dish, and stimulatory activities were calculated.
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2014-05-15
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