Human resident memory T cells exit the skin and mediate systemic Th2-driven inflammation.

To investigate the transcriptional profile of human circulating T cells with skin tissue-resident memory phenotype (cTRM) in GVHD we performed bulk RNA sequencing from SMART-seq2 of sorted human cTRM (CD3+ CD4+ CLA+ CD103+) and conventional T cells (CD3+ CD4+ CD103-), and single cell RNA sequencing from 10X Genomics of sorted human CD45+, both from isolated blood PBMCs of patients with active acute GVHD. Frozen PBMC isolated of whole blood from patients with active acute GVHD were thawed, stained for FACS-sorting and sorted for live CD45+ cells, which were submitted for 10X Genomics single-cell RNA sequencing (n=2, two replicates for donor 2), or sorted for live cTRM (live CD45+ CD3+ CD4+ CLA+ CD103) and Tconv (live CD45+ CD3+ CD4+ CD103-), wich were submitted for SMART-seq2 bulk RNA sequencing (n=3). *** Submitter Comment: Due to security issue we are not submitting the raw data ***