Swine strains were cultured overnight at 37C with shaking at 220 rpm in lysogeny broth (LB) (Thermo Fisher Scientific, Asheville, NC, USA). To maximize total genomic DNA (gDNA) yields, bacterial overn
We developed a PCR-based open reading frame typing (POT) method to genotype a broad range of STEC serotypes. To select detection ORFs for POT, we used both genome data from our STEC isolates and publi
Distribution of putative virulence genes among Shiga toxin-producing Escherichia coli (STEC) isolates with major/minor serotypes and O157/non-O157 isolates from patients.
1Enrichment broths were subjected to direct plating from July to December 2008 or to immunomagnetic separation (IMS) from January to June 2009. 2Sampling sites correspond to regions in the Culiacan Va