Catalysis of Ac-DEVD-AMC by procaspase-3 (dataset formatted for analysis by interferENZY)

<strong>Main description</strong> This dataset depicts the catalysis of the fluorogenic substrate Ac-DEVD-AMC to the fluorescent substrate AMC by recombinant procaspase-3 obtained in yeast cell extracts, for fixed concentration of enzyme and variation of concentration of initial substrate. It was originally documented in <em>Biophysical Chemistry 252 (2019) 106193</em> (https://doi.org/10.1016/j.bpc.2019.106193), and then used as a study case for the webserver interferENZY (a web-based tool for enzymatic assay validation and standardized kinetic analysis; visit https://interferenzy.i3s.up.pt for more information). To this end, it was converted to the format here presented: tab-separated *.txt input required for interferENZY analysis. <strong>Dataset organization</strong> Line 1: Tab-separated initial concentrations of substrate Ac-DEVD-AMC in micromolar (µM) concentration Line 2: Concentration of protein in yeast extract (0.123 mg/mL) Line 3: Units of time Line 4: Units of concentration for substrate values and measurements Line 5: Dataset name Line 6 and downwards: Tab-separated column-pairs of the progress curves (time,Product) corresponding to the indicated values of initial concentrations of substrate in line 1 <strong>Contact information:</strong> Maria Filipa Pinto ([e-mail])<br> Pedro M. Martins ([e-mail]) i3S – Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal. Telephone number: [phone]主数据集描述：本数据集展示了酵母细胞提取物中获得的重组半胱天冬酶前体-3（procaspase-3）对荧光底物Ac-DEVD-AMC的催化反应，将其转化为荧光产物AMC；实验中固定酶浓度，改变初始底物浓度。该数据集最初发表于《Biophysical Chemistry》2019年第252卷，文章编号106193（https://doi.org/10.1016/j.bpc.2019.106193），后被用作interferENZY网页工具的研究案例——interferENZY是一款用于酶活实验验证与标准化动力学分析的在线工具，详细信息可访问https://interferenzy.i3s.up.pt。为适配该工具的分析需求，本数据集已转换为当前格式：即供interferENZY分析使用的制表符分隔*.txt输入文件。 数据集组织形式： 第1行：制表符分隔的初始底物Ac-DEVD-AMC浓度，单位为微摩尔（µM） 第2行：酵母提取物中蛋白质浓度（0.123 mg/mL） 第3行：时间单位 第4行：底物浓度值与检测值的浓度单位 第5行：数据集名称 第6行及后续行：制表符分隔的进度曲线列对（时间，产物浓度），与第1行所列的初始底物浓度一一对应。 联系方式： Maria Filipa Pinto（邮箱：[e-mail]）、Pedro M. Martins（邮箱：[e-mail]） 葡萄牙波尔图大学健康研究与创新研究所（i3S – Instituto de Investigação e Inovação em Saúde, Universidade do Porto），地址：Rua Alfredo Allen, 208, 4200-135 Porto, Portugal；联系电话：[phone]