Identification of the downstream target genes of miR-138 in gastric cancer

Our data showed that EIF4EBP1, NINJ1, CLIC3, STMN3, SPINK4, CD44, GSN, PRSS23 et al. were strongly downregulated by miR-138-mimics in gastric cancer (GC) cell lines. Besides, miR-138-5p has a profound effect on EMT signaling and proliferation signaling. In the EMT signaling, miR-138-5p negatively regulates the expression of SOX4, VIM, TGFB1, BMP1, ECM1 and CXCL8; In the cell proliferation signaling, miR-138-5p could negatively regulates the expression of PCNA, RHOC and CCND3. Overall design: To reveal the possible target genes of miR-138 in gastric cancer, we transfected miR-138-5p mimics in GC cells. The potent miR-138 target genes were identified by deep sequencing, using Illumina HiSeq 4000.