Molecular Architecture Of The Yeast Mediator Complex

The 21-subunit Mediator complex transduces regulatory information from enhancers to promoters, and performs an essential role in the initiation of transcription in all eukaryotes. This repository contains files used in the 3-D modeling of the entire Mediator complex, using an integrative modeling approach that combines information from chemical cross-linking and mass spectrometry; X-ray crystallography; homology modeling; and cryo-electron microscopy. The approach is validated by the use of X-ray crystal structures as internal controls and by consistency with previous results from electron microscopy and yeast two-hybrid screens. The model shows the locations and orientations of all Mediator subunits, as well as subunit interfaces and some secondary structural elements. Segments of 20–40 amino acid residues are placed with an average precision of 20 Å. The model reveals roles of individual subunits in the organization of the complex. For more information about how to reproduce this modeling, see https://salilab.org/mediator or the README file.由21个亚基组成的中介体复合物（Mediator complex）可将调控信息从增强子传递至启动子，并在所有真核生物的转录起始过程中发挥不可或缺的作用。本仓库包含用于完整中介体复合物三维建模的相关文件，所采用的整合建模方法整合了化学交联-质谱联用技术、X射线晶体学、同源建模以及冷冻电子显微术的相关数据。 该方法通过以X射线晶体结构作为内部对照，以及与既往电子显微镜和酵母双杂交筛选实验结果的一致性得到了验证。 该模型展示了所有中介体亚基的位置与取向，以及亚基相互作用界面与部分二级结构元件。长度为20至40个氨基酸残基的肽段的定位平均精度可达20埃。 该模型还揭示了单个亚基在复合物组装过程中所发挥的作用。 如需了解该建模流程的复现方法，请访问 https://salilab.org/mediator 或查阅README文件。