Epigenetic Rewiring of Metastatic Cancer to the Brain: Focus on Lung and Colon Cancers

Genome wide DNA methylation profiling of brain metastasis from colorectal and lung cancer. The Illumina Infinium MethylationEPIC was used to obtain DNA methylation profiles across approximately 850,000 CpGs in brain metastasis samples. Samples included 1 breast ductal invasive carcinoma, 4 colon adenocarcinoma, 1 melanoma, 1 multiple mieloma, 7 non small cell lung cancer adenocarcinoma, 3 non small cell lung cancer G3, 4 non small cell lung cancer SCC, 1 prostate cancer adenocarcinoma and 1 serous carcinoma. Bisulfite-converted DNA was amplified, fragmented and precipitated. The DNA was subsequently hybridized to probes on the BeadChips and scanned using the NextSeq 550 System (Illumina).