Oxygen consumption rates/zero valen iron dissolution of FeOB (Ferrozine assay) with Kanamycin addition

<p>In these experiments, oxygen consumption rates were measured&nbsp;for the iron-oxidizing bacteria (FeOB)&nbsp;Mariprofundus ferrooxydans PV-1 as well as the neutrophilic FeOB \"TAG-1\" (An isolate from hydrothermal vents in the Mid Atlantic ridge that has not yet been formally named. TAG simply refers to the TAG hydrothermal site from which this microbe was isolated.). These experiments measure oxygen consumption at 100 uM oxygen at 20 degrees C. After 3 days the media in each vessel was replaced with fresh media containing kanamycin to stop any further growth and examine abiotic effects of iron mat production on zero valent iron dissolution.</p>

本实验测定了铁氧化细菌（Iron-oxidizing Bacteria，FeOB）Mariprofundus ferrooxydans PV-1以及嗜中性铁氧化细菌“TAG-1”的氧气消耗速率，实验条件为100 μM氧气浓度、20摄氏度。其中，“TAG-1”为分离自中大西洋脊热液喷口的暂未正式命名的菌株，“TAG”即指代分离该微生物的TAG热液位点。培养3天后，将各反应容器内的培养基更换为添加卡那霉素（Kanamycin）的新鲜培养基，以终止菌体的进一步生长，并探究铁垫（Iron mat）形成对零价铁（Zero Valent Iron）溶解的非生物影响。