Data for "Neural space-time model for dynamic multi-shot imaging"
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Data for "Neural space-time model for dynamic multi-shot imaging" Link to preprint: https://www.biorxiv.org/content/10.1101/2024.01.16.575950 Code: https://github.com/rmcao/nstm Raw images from structured illumination microscopy (SIM): beads.tif: a dense microbead sample with vibrating motion (Fig.2) mito.tif: a live RPE-1 cell expressing StayGold-tagged mitochondrial matrix protein (Fig.3) er.tif: a RPE-1 cell expressing StayGold-tagged endoplasmic reticulum (Fig.4) f-actin.tif: a live RPE-1 cell tagged with F-Actin Halo-JF585 (Extended Data Fig.6) f-actin_extradelay.tif: a live RPE-1 cell tagged with F-Actin Halo-JF585 with extra time delay between rotations (Extended Data Fig.6) Baseline reconstruction: beads_fairSIM.tif: conventional reconstruction of the dense microbead sample using fairSIM software (https://www.fairsim.org) mito_cudasirecon.tif: conventional reconstruction for a mitochondria-labeled cell (mito.tif) using cuda-accelerated SIM reconstruction software (https://github.com/scopetools/cudasirecon) er_cudasirecon.tif: conventional reconstruction for an endoplasmic reticulum-labeled cell (er.tif) using cuda-accelerated SIM reconstruction software f-actin_cudasirecon.tif: conventional reconstruction for a F-Actin Halo-JF585 labeled cell (f-actin.tif) using cuda-accelerated SIM reconstruction software f-actin_extradelay_cudasirecon.tif: conventional reconstruction for a F-Actin Halo-JF585 labeled cell with extra delay (f-actin_extradelay.tif) using cuda-accelerated SIM reconstruction software
本数据集对应论文《用于动态多帧成像的神经时空模型》(Neural space-time model for dynamic multi-shot imaging)。 预印本链接:https://www.biorxiv.org/content/10.1101/2024.01.16.575950 代码仓库:https://github.com/rmcao/nstm 结构照明显微镜(structured illumination microscopy, SIM)原始图像: beads.tif:携带振动运动的密集微球样本(对应图2) mito.tif:表达经StayGold标记的线粒体基质蛋白的活RPE-1细胞(对应图3) er.tif:表达经StayGold标记的内质网的RPE-1细胞(对应图4) f-actin.tif:经F-Actin Halo-JF585标记的活RPE-1细胞(对应扩展数据图6) f-actin_extradelay.tif:旋转间隔带有额外时间延迟的、经F-Actin Halo-JF585标记的活RPE-1细胞(对应扩展数据图6) 基准重建结果: beads_fairSIM.tif:采用fairSIM软件对该密集微球样本完成的传统重建(https://www.fairsim.org) mito_cudasirecon.tif:采用cuda加速的SIM重建软件,对线粒体标记细胞(mito.tif)完成的传统重建(https://github.com/scopetools/cudasirecon) er_cudasirecon.tif:采用cuda加速的SIM重建软件,对内质网标记细胞(er.tif)完成的传统重建 f-actin_cudasirecon.tif:采用cuda加速的SIM重建软件,对F-Actin Halo-JF585标记细胞(f-actin.tif)完成的传统重建 f-actin_extradelay_cudasirecon.tif:采用cuda加速的SIM重建软件,对带有额外旋转延迟的F-Actin Halo-JF585标记细胞(f-actin_extradelay.tif)完成的传统重建



