Xaira-Therapeutics/X-Atlas-Pisces

--- license: cc-by-nc-sa-4.0 --- # X-Atlas/Pisces X-Atlas/Pisces is a Perturb-seq atlas containing 25.6 million perturbed single-cell transcriptomes across 16 biologically diverse contexts, including widely used cell lines (HCT116, HEK293T, HepG2), induced pluripotent stem cells (iPSCs), resting and CD3/CD28 activated Jurkat T lymphoma cells, and multi-lineage differentiating iPSCs.  **(Coming Soon)** The following data will be uploaded to this dataset: 1. All cells from X-Atlas/Orion (cells with a valid dual-guide pair from the **HCT116** and **HEK293T** screens) 2. Cells containing a valid dual-guide pair AND perturbations in the 200-gene validation set OR a non-targeting control from the **HepG2** and **iPSC** screens 3. All cells with a valid dual-guide pair from the **Jurkat Resting** and **Jurkat Active** screens--- 许可证：CC BY-NC-SA 4.0（知识共享署名-非商业性使用-相同方式共享4.0国际协议） --- # X-Atlas/Pisces X-Atlas/Pisces是一款扰动测序（Perturb-seq）数据集图谱，涵盖16种生物学背景各异的实验体系中的2560万条经基因扰动的单细胞转录组数据，实验体系包括常用细胞系（HCT116、HEK293T、HepG2）、诱导多能干细胞（induced pluripotent stem cells，iPSCs）、静息态与经CD3/CD28激活的Jurkat T淋巴瘤细胞，以及多谱系分化诱导多能干细胞。  **【即将上线】** 本数据集将新增以下数据： 1. 来自X-Atlas/Orion的全部细胞（即**HCT116**与**HEK293T**筛选实验中带有有效双向导RNA对（dual-guide pair）的细胞） 2. 带有有效双向导RNA对且在200基因验证集存在基因扰动，或来自**HepG2**与**iPSC**筛选实验的非靶向对照细胞 3. 来自**静息Jurkat**与**激活Jurkat**筛选实验的全部带有有效双向导RNA对的细胞