Single-cell RNA-Seq of human primary lung and bronchial epithelium cells

This dataset contains count matrices and per-cells metadata tables for RNA sequencing of 39778 single nuclei from healthy primary lung samples of 12 lung adenocarcinoma patients as well as 17451 single human bronchiole epithelial cells from 4 donors. All samples were processed using the 10X Genomics Chromium platform with v2 chemistry and sequenced with one sample per lane on an Illumina HiSeq4000. Reads were aligned to the hg19 reference genome version 1.2.0 obtained from 10X Genomics. Data processing was performed using Seurat3.
The metadata table includes patient ID, sex, age, smoking status, and cell type, as well as QC statistics (number of genes, number of cells, ratio of mitochondrial reads).

本数据集包含两类测序数据：其一为12名肺腺癌患者健康原发性肺组织样本的39778个单细胞核RNA测序计数矩阵及单细胞元数据表；其二为4名捐赠者的17451个人类细支气管上皮单细胞数据。所有样本均采用10X基因组学(10X Genomics) Chromium平台搭配v2化学试剂进行处理，随后在Illumina HiSeq4000测序仪上以每个泳道加载一个样本的方式完成测序；测序reads比对至从10X基因组学获取的hg19参考基因组版本1.2.0，数据处理工作通过Seurat3完成。
元数据表包含患者ID、性别、年龄、吸烟状态、细胞类型，以及质控统计指标（基因数量、细胞数量、线粒体reads占比）。