Transcriptomic analysis of mouse hepatic progenitors expressing different level of the HRasG12V oncogene (in vivo and ex vivo).

Analysis was done in parrallel on tissue culture and on orthotopic tumor xenografts from primary (liver) and metastatic (peritoneum) site after separation of tumoral and stromal cells components. mRNA profiles of BMEL cells transduces with an empty vector or expressing eitheir low (RasLOW) or high (RasHIGH) levels of the HRasG12V oncogene were generated by deep sequencing, in triplicate, using Illumina HiSeq2500. mRNA profiles of tumor and stromal cells after dissociation and isolation from liver xenograft or peritoneal metastasis from 4 independant mice were generated using Illumina HiSeq2500