Table 2 in Genomic data mining approaches for the discovery of anticancer peptides from Ganoderma sinense

Table 2 Sequence alignment results of trypsin-digested peptides with known ACPs and the properties of these peptides. G. sinenseTrypsin-digested PeptidesHitsGrand average ofThe totalEstimated half-life(hours,Proteome(Acc)bhydropathicitynet chargemammalian reticulocytes, inNumbervitro)2–1aMPHLILSCSR1(88.3)0.56+1302–2aEHLPPWVLHQR1(100)0.927016VFPTTQGWVR1(33.3)0.11+110011MGAIFSAIGSAINAVVYAVADIIMIIVSVIVTIIVTIFDIIFDILCCNCFGGR2(100;1.9023045.5)14MLPVVVQNIIK2(36.4;1.35+13045.5)15YTALLGGVFYGIVHR2(83.3;0.86+12.838.4)17VLVHTVAGGLGL1(44.4)1.725010018VCVIGAGASGMAAAYALSK1(53.3)1.26+110020IAVAGAGLSAASGIPTFR1(100)1.07+12021FPGMLPGR1(71.4)0+11.122YSTVPGTCIR1(42.6)0.12+12.824INDVVMGTGTGSQK1(71.4)0.2102029ALPQPDLGLWLLQR1(71.4)0.0904.431GWWWWCPSSECECR1(54.5)0.813033NDFVVPCPR1(44.4)0.1101.4 a Proteome No. 2 has two cleaved peptides predicted to be anticancer peptides. b Hits represent the number of anticancer peptides containing similar sequences, and Acc represents the similarity.表2 已知抗肿瘤肽（Anticancer Peptides, ACPs）与胰蛋白酶消化肽的序列比对结果及此类肽段的相关性质 G. sinense 胰蛋白酶消化肽 肽段编号 肽序列 Hits(相似度Acc) 总平均亲水性 净电荷 体外哺乳动物网织红细胞预估半衰期(小时) 蛋白质组编号 2–1a MPHLILSCSR 1(88.3) 0.56 +1 30 2 2–2a EHLPPWVLHQR 1(100) 0.92 70 16 VFPTTQGWVR 1(33.3) 0.11 +1 100 11 MGAIFSAIGSAINAVVYAVADIIMIIVSVIVTIIVTIFDIIFDILCCNCFGGR 2(100;1.90) 230 45.5 MLPVVVQNIIK 2(36.4;1.35) +1 30 45.5 YTALLGGVFYGIVHR 2(83.3;0.86) +1 2.8 38.4 VLVHTVAGGLGL 1(44.4) 1.72 50 100 VCVIGAGASGMAAAYALSK 1(53.3) 1.26 +1 100 IAVAGAGLSAASGIPTFR 1(100) 1.07 +1 20 FPGMLPGR 1(71.4) 0 +1 1.1 YSTVPGTCIR 1(42.6) 0.12 +1 2.8 INDVVMGTGTGSQK 1(71.4) 0.21 0 20 ALPQPDLGLWLLQR 1(71.4) 0.09 0 4.4 GWWWWCPSSECECR 1(54.5) 0.81 30 NDFVVPCPR 1(44.4) 0.11 0 1.4 注a：编号为2的蛋白质组含有2条经预测为抗肿瘤肽的酶切肽段。 注b：Hits指代含相似序列的抗肿瘤肽的数量，Acc则代表序列相似度。