Epigenetic analysis of CD8 T cells in absence of IL-10R signaling in murine tumor

ATAC-seq analysis of CD8 T cell subsets. The goal of this study was to understand the chromatin states associated to the effect of IL-10 in preventing activation-induced exhaustion of CD8+ T cells in the context of mouse tumor. The chromatin accessibility changes induced upon a-IL-10R antibody treatment in tumor-bearing mice were studied using ATACseq. Overall design: C57BL/6 mice were transplanted with splenocytes from leukemic TCL1 mice, and after three weeks randomized to treatment with aIL-10R blocking antibody (n=3) or the corresponding isotype control (n=3). CD8+ T cells were obtained from spleen, cell subsets (naive, memory, effector PD1int and PD1hi) were sorted by flow cytometry and ATAC-seq was performed. Additional naive CD8+ T-cell samples (n=3) were sorted from non-tumor bearing WT mice.