LC-MS raw data of Aβ40 and medin co-aggregation

This repository contains raw LC–MS data files (.lcd format) generated from analyses of Aβ40, Medin, and their co-aggregation samples. These datasets correspond to the experiments presented in Figure 1 and Supplementary Figure 2 of the associated manuscript. The files are provided in Shimadzu proprietary .lcd format. LC–MS Instrumentation and Methods Liquid chromatography analysis was performed using a Shimadzu Prominence LC-30 system coupled to a Zorbax 300 Extend-C18 analytical column (2.1 × 100 mm, 3.5 µm, 300 Å) with a matching narrow-bore guard column (2.1 × 12.5 mm, 5 µm). Mass spectrometric detection was carried out online using a Shimadzu LCMS-2020 single quadrupole detector operating in electrospray ionization (ESI) mode. Chromatographic separation was conducted at 80°C with a flow rate of 0.35 mL/min, using a binary solvent system consisting of: Solvent A: 0.1% formic acid in water Solvent B: 0.07% formic acid in acetonitrile Detection was achieved using a photodiode array (PDA) detector at 214 nm. Acquisition Details Software: Shimadzu LabSolutions v5.97 SP1 File format: .lcd (Shimadzu proprietary format) Chromatogram type: MultiChrom Time range: 0–19.008 min Sampling interval: 640 ms Intensity units: mAU Experimental Context LC-MS data for the following peptides in 50 mM Tris (pH 7.4), 100 mM NaCl, and 10 µM ThT buffer 20 µM Aβ40 peptide 20 µM Medin peptide 20 µM Aβ40–Medin co-aggregation samples本仓库包含源自Aβ40、Medin及其共聚集样品分析所得的原始液相色谱-质谱联用（LC–MS）数据文件（格式为.lcd）。这些数据集对应于相关论文中图1及补充图2所呈现的实验内容。文件以岛津（Shimadzu）专属的.lcd格式存储。 LC-MS仪器与实验方法 液相色谱分析采用岛津Prominence LC-30系统，搭配Zorbax 300 Extend-C18分析柱（2.1 × 100 mm，3.5 µm，300 Å）及配套窄口径保护柱（2.1 × 12.5 mm，5 µm）。 质谱检测采用岛津LCMS-2020单四极杆检测器在线完成，运行模式为电喷雾电离（ESI）。 色谱分离在80℃条件下进行，流速为0.35 mL/min，采用二元溶剂体系，具体组分如下： 溶剂A：含0.1%甲酸的水溶液 溶剂B：含0.07%甲酸的乙腈溶液 采用光电二极管阵列（PDA）检测器在214 nm波长下完成检测。 采集参数详情 软件：岛津LabSolutions v5.97 SP1 文件格式：.lcd（岛津专属格式） 色谱图类型：MultiChrom 时间范围：0–19.008 min 采样间隔：640 ms 强度单位：mAU 实验背景 本数据集对应的样品均配制于50 mM Tris（pH 7.4）、100 mM NaCl及10 µM硫黄素T（ThT）缓冲液中，具体如下： 20 µM Aβ40肽样品 20 µM Medin肽样品 20 µM Aβ40与Medin的共聚集样品