Rating of obtained polymerase chain reaction (PCR) products.

Rating of the PCR products obtained from using the degenerate oligonucleotide primers shown in Table 3 to amplify ten target genes in six apocritan Hymenoptera. ++  =  target PCR product in excess. +  =  target PCR product sufficient for direct sequencing. +/−  =  target PCR product insufficient for direct sequencing. –  =  no target PCR product. (?)  =  unclear whether or not PCR products include amplicon of target gene.
*Secondary PCR amplification product likely hampering direct sequencing.