SAIMOS - Biological and Flow Cytometry data collected from CTD stations in South Australia, in February 2012

Flow cytometry data was collected in February 2012, in waters off South Australia. The general purpose of the study is to be able to establish background knowledge on the ecosystem on the continental shelf of South Australia and the impact of upwelling/saline outflow events on microbial communities to ultimately develop a biogeochemical model of the region. Sampling was carried out during cruises conducted on board the RV Ngerin as part of the Southern Australian Integrated Marine System (SAIMOS). During each cruise, the physical, chemical and biological properties of the chlorophyll fluorescence maximum (FM) layer were investigated. Flow cytometry data has been collected for picophytoplankton, bacteria and viruses.Six main stations have been sampled over the course of the study, five are located on the 100 m isobath, i.e. RS (35.508S, 136.278E), B2 (35.418S, 136.148E), B3 (35.258S, 136.048E), SAM2CP/B4 (35.168S, 135.418E) and SAM5CB/B5 (35.008S, 135.198E), and one from an offshore station (B1; 36.188S, 136.178E) located southwest of Kangaroo Island. Note that combining the distances between stations (14–25 nautical miles), the average component of the current velocity at middepth along the shelf (0.01 m s21) and the average speed of the vessel (i.e. 9 knots) indicate that different water masses were sampled at each station. Additional samples have on occasion been collected from the National Reference Station (NRS) at Kangaroo Island (35.832S, 136.447E) and the SA Spencer Gulf Mouth Mooring (SAM8SG, 35.25S, 136.690E), where the saline outflow occurs.

本数据集的流式细胞术（Flow Cytometry）数据采集于2012年2月，采集区域为澳大利亚南部外海。本研究的核心目标为：厘清澳大利亚南部大陆架海域的生态系统本底信息，阐明上升流/高盐冲溢事件对微生物群落的影响，最终构建该区域的生物地球化学模型。本次采样依托“恩格林号”（RV Ngerin）科考航次开展，属于澳大利亚南部综合海洋系统（Southern Australian Integrated Marine System, SAIMOS）的研究内容之一。每航次均针对叶绿素荧光最大值（Chlorophyll Fluorescence Maximum, FM）层的物理、化学与生物学特性展开调查。本数据集的流式细胞术数据覆盖超微型浮游植物、细菌与病毒三类研究对象。本研究共布设6个主要采样站位：其中5个站位位于100米等深线处，分别为RS（35.508°S，136.278°E）、B2（35.418°S，136.148°E）、B3（35.258°S，136.048°E）、SAM2CP/B4（35.168°S，135.418°E）与SAM5CB/B5（35.008°S，135.198°E）；剩余1个为离岸站位B1（36.188°S，136.178°E），位于袋鼠岛西南海域。需说明的是：结合站位间距（14~25海里）、陆架中层海流的平均流速分量（0.01 m·s⁻¹）与科考船平均航速（9节），可推断各站位所采集的水体团互不相同。此外，研究团队还于袋鼠岛国家参考站（National Reference Station, NRS，35.832°S，136.447°E）以及发生高盐冲溢事件的南澳斯宾塞湾口锚泊站（SAM8SG，35.25°S，136.690°E）采集了补充样本。