Evidence for Laboratory Contamination in DNA Sequences from Alleged Tridactyl Specimens from Nazca, Peru - supplementary materials

Supplementary data for the study analyzing DNA sequences from alleged ancient "tridactyl" (three-fingered) specimens reportedly found in the Nazca region of Peru. This dataset contains all processed data required to reproduce the genetic analysis demonstrating that:1. The sequenced DNA is unambiguously modern human (Homo sapiens)2. No authentic ancient DNA damage patterns are present3. Laboratory contamination is the most likely source of the genetic signal CONTENTS: vcf/ - Variant call files (VCF format, gzipped) Aligned to human_g1k_v37 (hg19/GRCh37) using GATK UnifiedGenotyper. Includes ancient0002-0004 (NCBI SRA) and momia1,3,5,6 specimens. multiqc_reports.zip - Quality control reports MultiQC reports from nf-core/eager ancient DNA pipeline processing. Includes alignments to hg38 (nuclear) and rCRS (mitochondrial) reference genomes. Also includes Chachapoya control sample (NA50precapture, SRR1298755) for comparison. haplogroup_results/ - Haplogroup classifications - Mitochondrial DNA haplogroups from Haplogrep3 (PhyloTree Build 17) - Y-chromosome haplogroup from Yleaf v2 (ISOGG 2023) checksums.md5 - MD5 checksums for data integrity verification ORIGINAL SEQUENCE DATA:- ancient0002: NCBI SRA SRR21031366- ancient0003: NCBI SRA SRR20755928- ancient0004: NCBI SRA SRR20458000- NA50precapture (control): NCBI SRA SRR1298755 (BioProject PRJNA246597) REFERENCE PANEL:Allen Ancient DNA Resource (AADR) v62.0本数据集为某项研究的补充数据，该研究针对据称在秘鲁纳斯卡地区发现的疑似古代“三趾”（三指）标本的DNA序列展开分析。 本数据集包含复现该遗传分析所需的全部经过处理的数据，可验证以下结论：1. 经测序的DNA明确归属现代智人（Homo sapiens）；2. 未检测到真正的古代DNA损伤特征；3. 实验室污染是该遗传信号最可能的来源。 数据集内容： vcf/ 目录：变异识别文件（Variant Call Format，VCF格式，已gzip压缩）。采用GATK UnifiedGenotyper工具将测序数据比对至人类参考基因组human_g1k_v37（hg19/GRCh37），涵盖ancient0002-0004（来自NCBI SRA数据库）及momia1、3、5、6号标本。 multiqc_reports.zip：质量控制报告。包含nf-core/eager古代DNA流程生成的MultiQC报告，涵盖比对至hg38（核基因组）与rCRS（线粒体参考基因组）的结果，同时附带查瓦波亚（Chachapoya）对照样本（NA50precapture，SRR1298755）用于对照分析。 haplogroup_results/ 目录：单倍群分类结果： - 线粒体DNA单倍群：基于Haplogrep3工具（PhyloTree Build 17）分析得到； - Y染色体单倍群：基于Yleaf v2工具（ISOGG 2023）分析得到。 checksums.md5：用于验证数据完整性的MD5校验和文件。 原始测序数据： - ancient0002：NCBI SRA编号SRR21031366 - ancient0003：NCBI SRA编号SRR20755928 - ancient0004：NCBI SRA编号SRR20458000 - NA50precapture（对照样本）：NCBI SRA编号SRR1298755（对应生物项目PRJNA246597） 参考面板：艾伦古代DNA资源（Allen Ancient DNA Resource, AADR）v62.0