CUT&RUN of PLAG1-S global chromatin occupancy in Lin-CD34+ cord-blood cells [Cut & Run]

To characterize genome-wide chromatin occupancy of the transcription factor PLAG1-S that could contribute to transient ex vivo expansion of human cord-blood (CB) derived HSC we performed CUT&RUN. 3 pools of Lin-CD34+ CB cells transduced to overexpress 3xFLAG-PLAG1-S were used for anti-FLAG or anti-IgG control enrichment of specific PLAG1-S DNA binding sites. Lin-CD34+ samples from 3 biologically distinct pools of CB were transduced to overexpress PLAG1-S, and 72 hours following viral introduction transduction marker positive cells were FACS-isolated for CUT&RUN and high-throughput sequencing.