WGS of Neisseria gonorrhoeae from clinical specimens. Clinical sample to genome: A novel approach for whole genome sequencing of Neisseria gonorrhoeae using target enrichment

Nucleic acid tests have largely replaced culture in many settings worldwide for detecting Neisseria gonorrhoeae, the causative agent of gonorrhoea. Consequently, whole genome sequencing, a critical component in molecular epidemiology, has become increasingly challenging because of the need for pure cultured bacteria. Here, we show that Agilent SureselectXT can be used to generate comparable sequence data directly from clinical samples as for cultured specimens.