Phylogenetic analysis of the TDRD7 LOTUS domain.

The evolutionary history for the OST-HTH/LOTUS domain shared by TDRD7 and Oskar proteins was inferred using the Neighbor-Joining method. The bootstrap consensus tree inferred from 2000 replicates is taken to represent the evolutionary history of the taxa analysed. Branches corresponding to partitions reproduced in less than 50% bootstrap replicates are collapsed. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (2000 replicates) are shown next to the branches. The evolutionary distances were computed using the Jones, Taylor & Thornton matrix-based method and are in the units of the number of amino acid substitutions per site. The analysis involved 12 amino acid sequences. All positions with less than 95% site coverage were eliminated. That is, fewer than 5% alignment gaps, missing data, and ambiguous bases were allowed at any position. There were a total of 81 positions in the final dataset. Evolutionary analyses were conducted in MEGA6.