Dataset: Method and timing of rhizobacteria inoculation to plant roots alters success and strength of aphid suppression

Insect pests cause substantial yield losses globally, necessitating novel pest control approaches beyond chemical pesticides. Rhizobacteria, beneficial root-associated bacteria, present a promising alternative by enhancing plant growth and defences against pests.
This study explored the use of sodium alginate encapsulation for precise inoculation of two rhizobacteria, Acidovorax radicis and Bacillus subtilis, to suppress aphid (Sitobion avenae) populations on barley (Hordeum vulgare). We optimized a method using 4mm diameter wet-matrix alginate beads for controlled bacterial delivery directly to plant roots.
Rhizobacteria inoculation via alginate beads at root emergence significantly reduced aphid reproductive output, outperforming seed soaking methods, with increased effects when plants were infested with a high initial density of aphids. This suggests the prolonged release from alginate beads led to more consistent bacterial establishment to enhance plant defence responses trigge..., This dataset includes results from experiments investigating the effects of root-inoculated bacteria on barley plants (Hordeum vulgare) and their interactions with aboveground aphid herbivores (Sitobion avenae). Two bacterial species, Acidovorax radicis N35 and Bacillus subtilis B171, were used, with a control treatment of 10mM MgCl2. Plants of two barley varieties (Barbarella and Firefoxx) were grown in Levington’s Advance F1 low nutrient compost. Bacterial cultures were prepared by growing on nutrient agar or broth, harvested, and resuspended at OD600=2.0. Barley seeds were surface-sterilized and inoculated either by soaking in bacterial solutions or embedding bacteria in alginate beads formed by dropping bacterial-alginate mixtures into CaCl2. 
Three fully-factorial experiments were conducted. The first compared seed soaking and bead inoculation methods on aphid numbers, measuring plant shoot and root length and aphid reproduction after two weeks. The second tested timing o..., , # Dataset: Method and timing of rhizobacteria inoculation to plant roots alters success and strength of aphid suppression

[https://doi.org/10.5061/dryad.ttdz08m85](https://doi.org/10.5061/dryad.ttdz08m85)

## *Principle Investigator Contact Information*

```
Name: Dr Sharon Zytynska
Institution: University of Liverpool, UK
Email: [e-mail]
```

## Description of the data and file structure

Data from three experiments is included in this submission, across five .csv files. Each experiment is fully factorial, with columns detailing the treatments used.

**Experiment 1:**Blenkinsopp_Expt_1_data.csv [contains all data needed to run associated R code]

**Experiment 2:** 

Blenkinsopp_Expt_2_data_totals.csv [data in wide-format, for analysis of totals]

Blenkinsopp_Expt_2_data_extended.csv [data in long-format, for analysis over time]

Blenkinsopp_Expt_2_data_survival.csv [data on survival for survival analyses]

**Experiment 3:** Blenkinsopp_Expt_3_data.csv [conta...,

昆虫害虫在全球范围内造成严重的产量损失，亟需开发超越化学农药的新型害虫防控策略。根际细菌（rhizobacteria）——一类定殖于植物根系的有益细菌——可通过促进植物生长并增强其抗虫能力，成为极具潜力的害虫防控替代方案。

本研究探索了利用海藻酸钠（sodium alginate）包埋技术，实现两种根际细菌的精准接种：食酸根瘤菌（Acidovorax radicis）与枯草芽孢杆菌（Bacillus subtilis），以抑制大麦（Hordeum vulgare）上的麦长管蚜（Sitobion avenae）种群。我们优化了一套方法，采用直径4mm的湿基质海藻酸钠微球，将细菌可控地投递至植物根系区域。

通过在植物根系萌发阶段使用海藻酸钠微球接种根际细菌，可显著降低麦长管蚜的繁殖量，其防控效果优于种子浸种法；且当植株初始蚜虫密度较高时，该处理的增效作用更为明显。这一结果表明，海藻酸钠微球的缓释特性可使细菌更稳定地定殖于植物根系，进而增强植物的防御反应trigge...

本数据集包含了探究根系接种细菌对大麦（Hordeum vulgare）及其与地上部植食性麦长管蚜（Sitobion avenae）相互作用的实验结果。实验所用的两种细菌分别为食酸根瘤菌N35（Acidovorax radicis N35）与枯草芽孢杆菌B171（Bacillus subtilis B171），并设置了10mM氯化镁（MgCl₂）作为空白对照。供试的两个大麦品种为Barbarella与Firefoxx，种植于Levington Advance F1低养分栽培基质中。细菌培养通过在营养琼脂或液体培养基中扩增获得，收集菌体后重悬至OD₆₀₀=2.0。大麦种子经表面灭菌后，采用两种方式接种细菌：一是将种子浸于细菌菌悬液中，二是将细菌-海藻酸钠混合液滴入氯化钙（CaCl₂）溶液中制备微球，通过包埋方式实现细菌接种。

本研究共开展3组全因子实验。第一组比较了种子浸种与微球接种两种接种方式对蚜虫种群数量的影响，并在接种两周后测量了植株地上部与根系长度以及蚜虫繁殖量。第二组测试了接种时机对防控效果的影响timing o...

# 数据集：根际细菌接种至植物根系的方法与时机调控蚜虫抑制效果的有效性与强度

[https://doi.org/10.5061/dryad.ttdz08m85](https://doi.org/10.5061/dryad.ttdz08m85)

## 项目负责人联系信息

姓名：Sharon Zytynska博士
任职机构：英国利物浦大学
邮箱：[e-mail]

## 数据与文件结构说明
本提交包含来自3组实验的数据，涵盖5个.csv格式文件。每组实验均采用全因子设计，数据列详细记录了所使用的实验处理方式。
**实验1：** Blenkinsopp_Expt_1_data.csv [包含运行关联R代码所需的全部数据]

**实验2：**
Blenkinsopp_Expt_2_data_totals.csv [宽格式数据，用于总量统计分析]
Blenkinsopp_Expt_2_data_extended.csv [长格式数据，用于时序动态分析]
Blenkinsopp_Expt_2_data_survival.csv [存活分析相关的蚜虫存活数据]

**实验3：** Blenkinsopp_Expt_3_data.csv [内容截断，原文此处为conta...]