Additional data for "Gametophyte thermal priming modifies the transcriptomic heat stress response of sporophyte progeny in Saccharina latissima"

Additional data and R scripts for the paper "Gametophyte thermal priming modifies the transcriptomic heat stress response of sporophyte progeny in Saccharina latissima" (not yet published). Experimental design and methodologies can be accessed at the paper's URL, available after its publication. Also available on GitHub: https://github.com/annemln/Slat_priming_transcriptomics Content: Metadata found in metadata.csv contains the treatment, time point and beaker ID for each sample. The scripts "slat_RNA_deseq2_rep.R" and "GO_slat_heatstress_ks.R" and input (count table from RNAseq and geneIDtoGO) to generate DEGs and GO enrichments, with a PCR plot. The data quality summary is an overview of generated reads and quality from Illumina sequencing data. geneIDtoGO is generated using annotation data from Denoeud et al., 2024, accessed from the Phaeoexplorer database: https://phaeoexplorer.sb-roscoff.fr/ Raw PAM (measurements for photosynthetic performance) data and R script "PAM_ANOVA_boxcox.R" for repeated measures ANOVA, with a boxplot. References: Denoeud, F., Godfroy, O., Cruaud, C., Heesch, S., Nehr, Z., Tadrent, N., Couloux, A., Brillet-Guéguen, L., Delage, L., Mckeown, D., Motomura, T., Sussfeld, D., Fan, X., Mazéas, L., Terrapon, N., Barrera-Redondo, J., Petroll, R., Reynes, L., Choi, S.-W., … Cock, J. M. (2024). Evolutionary genomics of the emergence of brown algae as key components of coastal ecosystems. Cell, 187(24), 6943-6965.e39.