Quartet of familiar males – 16p11.2 Del mouse strain – 2 WT + 2 Del/+ - M6
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We monitored the individual and social behaviours of each quartet of mice over three days and nights in the Live Mouse Tracker system (LMT, plugin 931; de Chaumont et al. 2019 Nat. Biomed. Engin.). This system tracks individually mice living in a group over several days and nights and extracts automatically the number, total duration and mean duration of more than thirty behavioural events describing the posture of the mouse, the types of social contacts, the dynamic social approach and escapes and complex social groupings (see de Chaumont et al. 2019 Nat. Biomed. Engin.). In this system, the four mice (10-14 weeks of age; 2 WT mice and 2 Del/+ mice) from the same housing cage (housed together from weaning on) were left undisturbed for 71 hours in a large transparent Plexiglas cage (50 x 50 x 40 cm), with fresh bedding, a house (width: 100 mm, depth: 75 mm, height: 40 mm) in red Plexiglas, 6 dental cotton rolls as well as food and water ad libitum. Light/dark cycle and temperature conditions were similar to those of the housing room (12/12h light/dark, lights on at 07:00 AM, 100 lux when the lights were on). Each recording session started between 03:00 and 04:00 PM. At the end of the session, mice were placed back in their home cage and the LMT setup was cleaned with soap water and dried with paper towels. The upload includes the sqlite database from LMT (processed).
本研究在活体小鼠追踪系统(Live Mouse Tracker, LMT,插件版本931;de Chaumont等,2019,《自然·生物医学工程》)中,对每笼四只小鼠的个体行为与社会行为开展了为期三昼夜的监测。该系统可对群居饲养的小鼠进行多昼夜的个体追踪,并自动提取三十余种行为事件的发生频次、总时长与平均时长,涵盖小鼠姿态、社会接触类型、动态社交趋近与回避行为,以及复杂的社交群体互动行为(详见de Chaumont等,2019,《自然·生物医学工程》)。 本实验所用4只小鼠均来自同一饲养笼(自断奶起即群居饲养),周龄为10~14周,其中2只为野生型(Wild Type, WT)小鼠,另外2只为Del/+小鼠。实验期间,这4只小鼠被置于尺寸为50×50×40 cm的大型透明亚克力(Plexiglas)饲养箱中,保持71小时不受干扰;箱内铺有新鲜垫料,配备1个红色亚克力(Plexiglas)材质的躲避屋(宽100 mm、深75 mm、高40 mm)、6个牙科棉卷,以及充足且可自由取用的食物与饮用水。实验环境的光照周期与温度与饲养室保持一致:采用12小时光照/12小时黑暗的循环节律,每日7:00开灯,光照时段内光照强度为100勒克斯。 每一轮记录均于当日15:00至16:00间启动。实验结束后,将小鼠放回原饲养笼,并用肥皂水清洁LMT实验装置,随后以纸巾擦干。本次上传内容包含经预处理的LMT系统生成的SQLite数据库文件。



